Strawberry Dna Extraction and Quantitative Hypothesis Development

We wanted to extract, see and analyze deoxyribonucleic acid from a single hemangioma simplex( 12. 11 g). The long, thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common, everyday materials. To start the surgery we needed the following supplies one strawberry, one plastic shape, 10 ml of Cell Lysis Buffer (10% detergent, 1% NaCl), one plastic zip-lock bag, one umber filter, and 25 ml of ice cold Ethanol.The first step of the assist was to obtain the clog of the plastic cup by itself, get a strawberry, remove the stem and leave, put it in the plastic cup and hence record the slant of plastic cup with strawberry. The results of this first step was weight of plastic cup 9. 63 g, weight of plastic cup and strawberry 21. 74 g. The following step was to place the strawberry in the zip-lock bag, nasty it and start smashing the strawber ry until it was completely broken up, the strawberry had a smoothie- deal look. 0 ml of Cell Lysis Buffer was added to the strawberry and gentle massaged it for or so one minute. This buffer, which has detergent in it, is to dissolve the cell and nuclear membranes and salts to switching the ionic bonds between the histones and DNA, it separates DNA from strawberry. During this step, my lab partner prepared the chocolate filter to filter the solution as determine by our instructor. We pullulate the cell lysate on the coffee filter and allowed it to go through it, to booster it, we used a spoon and gently pushed the substance to get the just about liquid out of it without braking the coffee filter.Before the filtration the texture looked solid, but after it looked analogous strawberry juice. Our instructor added 25 ml of ice cold fermentation alcohol to the cup and obtain the DNA. We could observe a solid white, mucus like material forming out of the juice. The excess of liqui d was removed from the cup, and the cup and DNA was weighted, resulting of 10. 81 g, which was subtracted from the cup weight, having 1. 18 g as the weight of DNA. After this, we calculated the % of the strawberry that was DNA. We concluded that the DNA from strawberry was 9. 74%